Farshad Darvishi - Faculty Member of Alzahra University

فارسی

Research Interest

My main interest is biotechnological and environmental applications of yeasts. My research group is active in the following areas:

New and powerful methods for strain characterization (X-Omics such as genomics, metagenomics, transcriptomics, proteomics, metabolomics, lipidomics, fluxomics) and improvement (such as metabolic engineering, reverse metabolic engineering, adaptive laboratory evolution (ALE), systems biology, synthetic biology).
Heterologous proteins expression in Saccharomyces cerevisiae, Yarrowia lipolytica, Pichia pastoris and other nonconventional yeasts.
Biotechnologically valuable products (such as lipase, laccase, glucose oxidase, asparaginase, glutaminase enzymes, and citric acid, isocitric acid, single cell protein, single cell oil,…) production using Yarrowia lipolytica from renewable sources and wastes.
Enzyme immobilization on nanomaterials (magnetic nanoparticles and nanocomposite) and its kinetics study
Microbial and bioelectricity production.
Isolation and biodiversity study of yeasts.

Refereed Journal Articles

Liu Z, Moradi H, Shi S, Darvishi F. 2021. Yeasts as microbial cell factories for sustainable production of biofuels. Renewable and Sustainable Energy Reviews, 143, 110907. DOI: 10.1016/j.rser.2021.110907
Fathi Z, Ribeiro Ramos Tramontin L, Ebrahimipour ‎G, Borodina I, Darvishi F. 2021. Metabolic engineering of Saccharomyces cerevisiae for production of β-carotene from hydrophobic substrates. FEMS Yeast Research, 21 (1), foaa068. DOI: 10.1093/femsyr/foaa068
Mazloum-Ravasan S, Madadi E, ‎Mohammadi A, Mansoori B, Mokhtarzadeh A, Baradaran B, Darvishi F. 2021. Yarrowia lipolytica L-asparaginase inhibits the growth and migration of lung (A549) and breast (MCF7) cancer cells. International Journal of Biological Macromolecules, 170, 406-414. DOI: 10.1016/j.ijbiomac.2020.12.141. ‎
Darvishi F. 2020. General and practical facts about the new Coronavirus (Covid-19). Iranian Biology Journal, 4 (7), 315-319.
Darvishi F. 2020. An overview of the world of viruses. Iranian Biology Journal, 3 (6), 35-43.
Mohammadi E, Ashengroph ‎M, Darvishi F. ‎2020. Development and evaluation of different strategies for the clean synthesis of silver nanoparticles using Yarrowia lipolytica and their antibacterial activity. Process Biochemistry, 94, 319-328. DOI: 10.1016/j.procbio.2020.03.024
Mazloum-Ravasan S, Madadi E, ‎ Fathi Z, Mohammadi A, Mosafer J, Mansoori B, Mokhtarzadeh A, Baradaran B, Darvishi F. 2020. The effect of Yarrowia lipolytica L-asparaginase on apoptosis induction and inhibition ‎of growth in Burkitt's lymphoma Raji and acute lymphoblastic leukemia MOLT-4 ‎cells. International Journal of Biological Macromolecules, 146,193-201. DOI: 10.1016/j.ijbiomac.2019.12.156
Darvishi F. 2019. Microbial hormones. Iranian Biology Journal, 3 (5), 54-60.
Fathi Z, Doustkhah E, Ebrahimipour ‎G, Darvishi F. 2019. Non-covalent versus metal-chelate based immobilization of Yarrowia lipolytica lipase on dendritic like-amino acid functionalized silica nanoparticles. Biomolecules, 9 (9), 502. DOI:10.3390/biom9090502
Darvishi F, Salmani A. 2019. The production of fatty acids with biofuel potential by Yarrowia lipolytica. Journal of Applied Biology. 32 (2), 174-185.
Darvishi F, Salmani A. 2019. Biovalorization of vegetable oil refinery wastewater into value-added ‎compounds by Yarrowia lipolytica. Journal of Chemical Technology and Biotechnology, 94 (9), 2961-2968. DOI: 10.1002/jctb.6102
Darvishi F. 2019. Pilot scale production of lipase powder by the improved spray-drying method. Nova Biologica Reperta, 6 (2), 261-267.
Darvishi F, Faraji N, Shamsi F. 2019. Production and structural modeling of a novel asparaginase in Yarrowia lipolytica. International Journal of Biological Macromolecules, 125, 955-961. DOI: 10.1016/j.ijbiomac.2018.12.162
Darvishi F, Abolhasan Moghaddami N. 2019. Optimization of an industrial medium from molasses for bioethanol production using the Taguchi statistical experimental-design method. Fermentation, 5 (1), 14. DOI: 10.3390/fermentation5010014
Darvishi F, Marzookian K, Tavvafi S. 2019. How to set up a biotech company. Iranian Biology Journal, 2 (3-4), 26-35.
Darvishi F, Moradi M, Madzak C, Jolivalt C. 2018. Laccase production from sucrose by recombinant Yarrowia lipolytica and its application to decolorization of environmental pollutant dyes. Ecotoxicology and environmental safety, 165, 278-283. DOI: 10.1016/j.ecoenv.2018.09.026
Darvishi F, Abolhasan Moghaddami N. 2018. Isolation of industrial strain Saccharomyces cerevisiae with high tolerance to ethanol from Iran's alcohol manufactures. Journal of Molecular and Cellular Research, 31 (4), 582-592.
Khadivi derakhshan F, Darvishi F, Dezfulian M, Madzak C. 2018. Bioinformatics study of expressed recombinant glucose oxidase in Yarrowia lipolytica and optimization of its production by Taguchi experiment design method. Journal of Microbial World, 11 (36), 217-229.
Darvishi F, Shamsi F. 2018. Investigation of the L-asparaginase production in the yeast Yarrowia lipolytica. Biological Journal of Microorganism, 7 (27), 73-79.
Darvishi F, Salmani A. 2018. The effect of olive oil on the production of omega fatty acids in Yarrowia lipolytica. Journal of Molecular and Cellular Research, 31 (3), 407-416.
Darvishi F, Zarei A, Madzak C. 2018. In silico and in vivo analysis of signal peptides effect on recombinant glucose oxidase production in nonconventional yeast Yarrowia lipolytica. World Journal of Microbiology and Biotechnology, 34: 128. DOI: 10.1007/s11274-018-2512-x
Darvishi F, Ariana M, Marella ER, Borodina I. 2018. Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals. Applied Microbiology and Biotechnology, 102: 5925–5938. DOI: 10.1007/s00253-018-9099-x
Fathi Z, Doustkhah E, Rostamnia S, Darvishi F, Ghodsi A, Yusuke Ide. 2018. Interaction of Yarrowia lipolytica lipase with dithiocarbamate modified magnetic carbon Fe₃O₄@C-NHCS₂H core-shell nanoparticles. International Journal of Biological Macromolecules, 117: 218-224. DOI: 10.1016/j.ijbiomac.2018.05.156
Zavareh S, Farrokhzad Z, Darvishi F. 2018. Modification of zeolite 4A for use as an adsorbent for glyphosate and as an antibacterial agent for water. Ecotoxicology and Environmental Safety, 155: 1-8. DOI: 10.1016/j.ecoenv.2018.02.043
Darvishi F. 2018. Use of Yarrowia lipolytica potentials for space travels. Iranian Biology Journal, 1 (2): 124-128.
Noora H, Shahabivand S, Karimi F, Aghaee A, Darvishi F. 2017. Optimization of growth and biomass production in the endophytic fungus Piriformospora indica. Journal of Molecular and Cellular Research, 30 (3): 441-451.
Darvishi F, Fathi Z, Ariana M, Moradi H. 2017. Yarrowia lipolytica as a workhorse for biofuel production. Biochemical Engineering Journal, 127: 87-96. DOI: 10.1016/j.bej.2017.08.013
Khadivi derakhshan F, Darvishi F, Dezfulian M, Madzak C. 2017. Expression and characterization of glucose oxidase from Aspergillus niger in Yarrowia lipolytica. Molecular Biotechnology, 59 (8): 307-314. DOI: 10.1007/s12033-017-0017-8
Darvishi F, Moradi M, Madzak C, Jolivalt C. 2017. Optimization of recombinant laccase production by Yarrowia lipolytica in a medium containing glucose as carbon source with Taguchi method. Biological Journal of Microorganism, 6 (23): 49-56. DOI: 10.22108/bjm.2017.21661
Heidarizadeh M, Doustkhah E, Rostamnia S, Fathi Rezaei P, Darvishi F, Zeynizadeh B. 2017. Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe₃O₄-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD. International Journal of Biological Macromolecules, 101: 696-702. DOI: 10.1016/j.ijbiomac.2017.03.152
Darvishi F, Moradi M, Madzak C, Jolivalt C. 2017. Production of laccase by recombinant Yarrowia lipolytica from molasses: Bioprocess development using statistical modeling and increase productivity in shake-flask and bioreactor cultures. Applied Biochemistry and Biotechnology, 181: 1228-1239. DOI: 10.1007/s12010-016-2280-8
Darvishi F. 2017. Screening of new microorganisms and their useful genes: From traditional methods to metagenomics. Iranian Biology Journal, 1 (1): 43-51.
Mahdavinia GR, Hosseini R, Darvishi F, Sabzi M. 2016. The release of cefazolin from chitosan/polyvinyl alcohol/sepiolite nanocomposite hydrogel films. Iranian Polymer Journal, 25 (11): 933–943. DOI: 10.1007/s13726-016-0480-2
Mahdavinia GR, Mousanezhad S, Hosseinzadeh H, Darvishi F, Sabzi M. 2016. Magnetic hydrogel beads based on PVA/sodium alginate/laponite RD and studying their BSA adsorption. Carbohydrate Polymers, 147: 379–391. DOI: 10.1016/j.carbpol.2016.04.024
Zavareh S, Zarei M, Darvishi F, Hasan Azizi. 2015. As(III) adsorption and antimicrobial properties of Cu-chitosan/alumina nanocomposite. Chemical Engineering Journal, 273: 610–621. DOI: 10.1016/j.cej.2015.03.112
Zavareh S, Darvishi F. Samandari G. 2015. Preparation and characterization of epoxy/oregano oil as a new epoxy-based coating material with both antimicrobial effect and increased toughness. Journal of Coatings Technology and Research, 12 (2): 610–621. DOI: 10.1007/s11998-014-9641-4
Darvishi F, Hosseini B. 2015. Investigation the effect of olive oil feeding strategies on Yarrowia lipolytica lipase production. Biological Journal of Microorganism, 4 (15): 1-8.
Darvishi F, Hosseini B, Fathirezaei P. 2015. Optimization of Yarrowia lipolytica lipase production by Taguchi experiment design method. Journal of Molecular and Cellular Research, 28 (3): 336-343.
Darvishi F, Hosseini B. 2015. Effect of olive oil with different purity grades on Yarrowia lipolytica lipase production. Biological Journal of Microorganism, 4 (13): 35-42.
Khodaei A, Arzanlou M, Babai-Ahari A, Darvishi F. 2014. Identification of black Aspergilli species on grape and raisin in Southern regions of East and West Azerbaijan Provinces. Journal of Applied researches in Plant Protection, 3(1): 49-64.
Darvishi F. 2013. Cloning and molecular analysis of extracellular lipase gene from native and mutant Yarrowia lipolytica strains. Jundishapur Journal of Microbiology, 6 (10): e8131. DOI: 10.5812/jjm.8131
Darvishi F, Destain J, Nahvi I, Thonart P, Zarkesh-Esfahani H. 2012. Effect of additives on freeze-drying and storage of Yarrowia lipolytica lipase. Applied Biochemistry and Biotechnology, 168: 1101–1107. DOI: 10.1007/s12010-012-9844-z
Darvishi F. 2012. Expression of native and mutant extracellular lipases from Yarrowia lipolytica in Saccharomyces cerevisiae. Microbial Biotechnology, 5 (5): 634-641. DOI: 10.1111/j.1751-7915.2012.00354.x
Mirbagheri M, Nahvi I, Emtiazi G, Mafakher L, Darvishi F. 2012. Taxonomic characterization and potential biotechnological applications of Yarrowia lipolytica isolated from meat and its products. Jundishapur Journal of Microbiology, 5 (1): 346-351. DOI: 10.5812/kowsar.20083645.2433
Mirbagheri M, Nahvi I, Emtiazi G, Darvishi F. 2011. Enhanced production of citric acid in Yarrowia lipolytica by Triton X-100. Applied Biochemistry and Biotechnology, 165: 1068-1074. DOI: 10.1007/s12010-011-9325-9
Darvishi F, Destain J, Nahvi I, Thonart P, Zarkesh-Esfahani H. 2011. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate. New Biotechnology, 28 (6): 756-760. DOI: 10.1016/j.nbt.2011.02.002
Darvishi F, Nahvi I, Zarkesh-Esfahani H. 2011. Lipase production by Yarrowia lipolytica from different carbon sources. Journal of Molecular and Cellular Research, 24 (2): 169-175.
Mirbagheri M, Nahvi I, Emtiazi G, Darvishi F. 2011. Isolation and identification of citric acid producing yeast to use in industry. JMBS, 2 (1): 77-89.
Darvishi F, Nahvi I, Zarkesh-Esfahani H. 2011. Investigation of nitrogen sources effect on lipase production of Yarrowia lipolytica yeast. JMBS, 2 (1): 45-53.
Mafakher L, Mirbagheri M, Darvishi F, Nahvi I, Zarkesh-Esfahani H, Emtiazi G. 2010. Isolation of lipase and citric acid producing yeasts from agro-industrial wastewater. New Biotechnology, 27 (4): 337-340. DOI: 10.1016/j.nbt.2010.04.006
Darvishi F, Nahvi I, Zarkesh-Esfahani H, Momenbik F. 2009. Effect of plant oils upon lipase and citric acid production in Yarrowia lipolytica yeast. BioMed Research International (Journal of Biomedicine and Biotechnology) 2009, 562943. DOI:10.1155/2009/562943
Darvishi F, Hojati Z, Motovali-bashi M. 2006. Rapid isolation and molecular detection of streptomycin-producing streptomycetes. JSSU, 14 (2): 51-55.
Darvishi F, Golbang N, Hojati Z, Motovali-bashi M. 2006. Isolation of the Streptomycin antibiotic production regulatory gene (strR) by PCR. Journal of Molecular and Cellular Research, 19 (3): 264-271.

Refereed conference articles (Oral and Poster)

Alavi A, Barzegari AA, ‎ Khalifehzadeh B, ‎Darvishi F. Antibacterial activity of Lactobacillus rhamnosus cell-free supernatant against Staphylococcus aureus and Escherichia coli. Iran’s 21st International Congress of Microbiology. 17-20 August 2020, Tehran, Iran.
Ghaffari M, Darvishi F, Nazeri N. Isolation of probiotic yeasts from local Hamedan dairy products. The 3rd International & 11th national congress of Biotechnology, 2019, Tehran, Iran.
Darvishi F, Moghaddami N. Isolation and identification of bioethanol producer Saccharomyces cerevisiae from Iran's alcohol producer manufactures. The 20th National and 8th International Congress of Biology. 22-24 August 2018, Maragheh, Iran.
Ashengroph ‎M, Darvishi F, Mohammadi E. Intra- and extra-cellular biosynthesis of silver ‎nanoparticles by Yarrowia lipolytica. The 20th National and 8th International Congress of Biology. 22-24 August 2018, Maragheh, Iran.
Darvishi F, Moghaddami N. Optimization of ethanol production by industrial strain of Saccharomyces cerevisiae. The 20th National and 8th International Congress of Biology. 22-24 August 2018, Maragheh, Iran.
Abbasi F, ‎ Abadi M, ‎Asadollahi M, ‎Darvishi F. Microbial production of xanthan gum by ‎Xanthomonas campestris using acorn starch as carbon source. The 20th National and 8th International Congress of Biology. 22-24 August 2018, Maragheh, Iran.
Darvishi F, Faraji N. Investigation of the L-Glutaminase production in the yeast Yarrowia lipolytica. The 19th National and 7th International Congress of Biology. 30 August-1 September 2016, Tabriz, Iran.
Darvishi F, Salmani N. Evaluation of single cell oil production in the yeast Yarrowia lipolytica. The 19th National and 7th International Congress of Biology. 30 August-1 September 2016, Tabriz, Iran.
Shamsi F, Darvishi F. Optimization of the L-Asparaginase production in the yeast Yarrowia lipolytica using Taguchi experimental design method. The 19th National and 7th International Congress of Biology. 30 August-1 September 2016, Tabriz, Iran.
Darvishi F, Shamsi F. Evaluation of the L-Asparaginase production in the yeast Yarrowia lipolytica. The 19th National and 7th International Congress of Biology. 30 August-1 September 2016, Tabriz, Iran.
Moradi M, Darvishi F, Madzak C, Jolivalt, C. Laccase production from sucrose by recombinant Yarrowia lipolytica. The 17th International & Iranian Congress of Microbiology. 23-25 August 2016, Tehran, Iran.
Khadivi derakhshan F, Darvishi F, Madzak C. Isolation and cloning of Aspergillus niger glucose oxidase in Yarrowia lipolytica expression monocopy vector. The 17th International & Iranian Congress of Microbiology. 23-25 August 2016, Tehran, Iran.
Moradi M, Darvishi F, Madzak C, Jolivalt, C. The use of recombinant laccase of Yarrowia lipolytica for decolorization of environmental pollutant dyes. The 17th International & Iranian Congress of Microbiology. 23-25 August 2016, Tehran, Iran.
Moradi M, Darvishi F, Madzak C, Jolivalt, C. Effect of different substrates on the heterologous production of a fungal laccase in a recombinant strain of Yarrowia lipolytica. The 16th International & Iranian Congress of Microbiology. 25-26 August 2015, Tehran, Iran.
Darvishi F. Effect of oleic and alkane compounds on POX2 promoter induction in Yarrowia lipolytica yeast. The 13th Iranian & the Second International Congress of Microbiology. 14-16 July 2012, Ardabil, Iran.
Mafakher L, Nahvi I, Zarkesh-Esfahani H, Mirolyaei M, Darvishi F. Candida galli, newly isolated lipolytic yeast from wastewater treatment plant of Isfahan. The 12th Iranian and 1st International Congress of Microbiology, May 2011, University of Kermanshah, Iran.
Darvishi F, Nahvi I, Ziaee S. Expression of native and mutant extracellular lipase from Yarrowia lipolytica in Saccharomyces cerevisiae. 6th conference on Recombinant protein production. 16 - 19 February 2011, Vienna, Austria.
Mirbagheri M, Nahvi I, Emtiazi G, Darvishi F. Isolation of native high-level citric acid producing yeasts. The 2nd national congress of biology young scientists, February 2011, Tehran, Iran.
Darvishi F, Destain J, Nahvi I, Thonart P, Zarkesh-Esfahani H. Effect of additives on freeze-drying and storage of Yarrowia lipolytica lipase. 14th International Biotechnology Symposium and Exhibition. 14 - 18 September 2010, Rimini, Italy. Published in Journal of Biotechnology, Vol.150, Supplement 1, November 2010, Page 352.
Mafakher L, Zarkesh-Esfahani H, Nahvi I, Mirolyaei M, Darvishi F. Study of lipolytic yeasts population on meat products. The 11th Iranian congress of Microbiology, April 2010, Lahijan, Iran.
Mafakher L, Darvishi F, Nahvi I, Zarkesh-Esfahani H, Mirolyaei M. Application of lipolytic yeasts for wastewater treatment plant. International conference on water & wastewater treatment, April 2010, University of Isfahan, Iran.
Darvishi F, Mafakher L, Nahvi I, Zarkesh-Esfahani H, Mirolyaei M. Investigation of lipase enzyme production by different strains of Yarrowia lipolytica. The 10th Iranian congress of Microbiology, 21 - 23 April 2009, Ilam, Iran.
Darvishi F, Hojati Z, Golbang N, Motovali-bashi M. Cloning of strR, a specific regulatory gene for the production of Streptomycin antibiotic into Escherichia coli. The 14th Iranian biology conference and the 2nd international conference of biology, 2006, University of Tarbiat Modares, Tehran, Iran.
Habibi E, Darvishi F, Ahmadi A. Nanostructures and drug delivery. The 2nd National Congress of biology students, 3 - 5 May 2006, Mashhad, Iran.
Habibi E, Darvishi F, Ahmadi A. Significance of synthetic nanostructures in dictating cellular response. The 2nd National Congress of biology students, 3 - 5 May 2006, Mashhad, Iran.
Habibi E, Darvishi F, Ahmadi A. Biomedical application of nanotechnology for drug targeting and gene therapy. The 2nd National Congress of biology students, 3 - 5 May 2006, Mashhad, Iran.
Darvishi F, Hojati Z, Motovali-bashi M. Isolation of the streptomycin-producing streptomycetes from Iranian soil in order to genetically modify them. The 13th Iranian biology conference and the first international conference of biology, 23 - 25 August 2005, Rasht, Iran.
Hojati Z, Golbang N, Darvishi F. Detection and isolation of the Streptomycin antibiotic production regulatory gene (strR) from Streptomyces griseus. The 7th Iranian congress of Microbiology, 2004, Semnan, Iran.
Hojati Z, Golbang N, Darvishi F, Motovali-bashi M. Investigation on the possibility of the streptomycin production regulatory gene in Escherichia coli. The 4th national congress of Biotechnology, 2004, Kerman, Iran.
Habibi E, Darvishi F. Nanotechnology for overcoming biological barriers. The 1st National Congress of biotechnology students, 15 - 17 December 2005, Tehran, Iran.
Hojati Z, Darvishi F. Genetic engineering of streptomycetes for novel generation antibiotics production. The 1st National Congress of biology students, 21 - 23 May 2003, Mashhad, Iran.
Emtiazi G, Darvishi F. Application of piezophilic bacteria in biotechnology. The 1st National Congress of biology students, 21 - 23 May 2003, Mashhad, Iran.
Darvishi F, Najafi F. Single cell protein production from rice bran by Saccharomyces cerevisiae. The 1st National Congress of biology and biotechnology students, 17 - 19 September 2002, Tehran, Iran.

Books

- Synthetic Biology of Yeasts: Tools and Applications. 2022. Springer. Heidelberg, Germany.

- Springer Major Reference Works (Encyclopedia) - Microbial Production of Food Bioactive Compounds. 2022. Springer. Cham, Switzerland.

- Biology and Biotechnology of Yarrowia lipolytica. 2019. Maragheh University Press. (In Persian)

- Microbial Fuels: Technologies and Applications. 2018. CRC Press – Taylor & Francis Group. Boca Raton, USA.
- Microbial Biotechnology: Progress and Trends. 2015. CRC Press – Taylor & Francis Group. Boca Raton, USA.
- Biotechnological Applications of the Yeast Yarrowia lipolytica. 2014. Springer. Cham, Switzerland.

- English for the Students of Microbiology. 2012. Maragheh University Press.

Book chapters

- Yarrowia lipolytica bioprocess development – From flask to ‎bioreactor. 2021. In: Blenner M, Wheeldon I (eds.). Yarrowia lipolytica: Methods and Protocols. Springer Protocols: Methods in Molecular Biology. Humana Press.

- Impressive potential of microorganisms to achieve the transition from fossil fuels to ‎biofuels. 2018. In: Darvishi F, Hilligsmann S (eds.). Microbial Fuels: Technologies and ‎Applications. CRC Press – Taylor & Francis Group. Boca Raton, USA.

- Microbial biotechnology: An introduction. 2015. In: ‎Darvishi F, Chen H‏.‏‎ ‎ (eds.). Microbial ‎Biotechnology: Progress and Trends. CRC Press – Taylor & Francis Group. Boca Raton, USA.

- Microbial Biotechnology in Olive Oil Industry. 2012. In: Dimitrios B (ed.). Olive Oil - ‎Constituents, Quality, Health Properties and Bioconversions. InTech. Rijeka, Croatia.

Patents